Potential of multi-strain probiotics extract as an anti-inflammatory agent through inhibition of macrophage migration inhibitory factor activity
نویسندگان
چکیده
Background: Probiotics are reported to have a role for improving health conditions and reduce the risk of diseases associated with inflammation. However, how they affect inflammation has not been well studied. The occurs during progression chronic could damage normal function cells, tissues, organs. Macrophage migration inhibitory factor (MIF) is recognized as cytokine playing key in process. Inhibition its activity used by researchers an approach alleviating This study aims evaluate potential effect extract multi-strain probiotics consisting Lactobacillus casei EMRO 002, L. 213, plantarum 009, fermentum 211, rhamnosus 014, bulgaricus 212,and Rhodopseudomonas palustris 201 on MIF tautomerase activity, reversibility, mechanism inhibition. Hence, scope this evaluation functional characteristics activity.Methods:The were centrifuged supernatant was separated from pellet. supernatant, i.e. extract, mixed analyzed inhibition activity. reversibility evaluated preincubation dilution assays, determined kinetic evaluation.Results:The inhibited IC50 7.80 ± 1.96 mg/L. assays showed that reversible, predicted components might bind enzyme active site other site(s) MIF.Conclusion:The findings show able inhibit reversibly suggest be anti-inflammatory agent treatment inflammation.Keywords: Multi-strain probiotics, anti-inflammatory, macrophage factor, reversible inhibition, evaluation.
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ژورنال
عنوان ژورنال: Functional Foods in Health and Disease
سال: 2023
ISSN: ['2160-3855', '2378-7007']
DOI: https://doi.org/10.31989/ffhd.v13i1.1033